半岛bd体育手机客户端 说明

一般描述

Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

特异性

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

免疫原

Purified mouse IgG, Fc fragment

应用

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37℃. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37℃.

其他说明

Antibody adsorbed with human IgG and rat serum proteins.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

制备说明

Adsorbed to reduce background staining with human or rat samples.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00162626
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	goat
偶联物	alkaline phosphatase conjugate
抗体形式	affinity isolated antibody
antibody product type	secondary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
technique(s)	direct ELISA: 1:40,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)