半岛bd体育手机客户端 说明
一般描述
Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON?X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.
Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON?X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.
Conductivity (1.0 M, 24°C): <100 ?mhos.
包装
1 g in Glass bottle
25, 250 g in Glass bottle
1 kg in Glass bottle
5, 10 g in Plastic ampoule
应用
CHAPS 是一种用于膜生物化学的非变性两性离子洗涤剂。
可用于溶解膜蛋白以及打断蛋白-蛋白相互作用。CHAPS 具有低胶束分子量 (6,150) 和高临界胶束浓度 (6-10mM)，因此可通过透析从样品中除去。它也适合在等电聚焦和二维电泳中用于溶解蛋白质。CHAPS 常用于非变性(无尿素)等电聚焦，已发现它对某些亚细胞制备物和植物蛋白能够得出优异的分辨率。通常将介于 2-4% (w/v) 间的浓度用于等电聚焦凝胶。
警告
Toxicity: Harmful (C)
重悬
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
其他说明
Chen, Y., et al. 1992.Biochemistry31,2415.
Ofri, D., et al. 1992.J. Neurochem.58,628.
Ransom, R.W., et al. 1992.Biochem. Pharmacol.43,1823.
Simonds, W.F., et al. 1992.Proc. Natl. Acad. Sci. USA77,4623.
Yannariello-Brown, J., and Weigel, P.H. 1992.Biochemistry31,576.
Hjelmeland, L.M. 1980.Proc. Natl. Acad. Sci. USA77,6368.
Simonds, W.F., et al. 1980.Proc. Natl. Acad. Sci. USA77,4623.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
基本信息
| 经验(实验)分子式 | C32H58N2O7S |
| 分子量 | 614.88 |
| MDL编号 | MFCD00012116 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥98% (NMR) |
| 形式 | crystalline powder |
| 分子量 | micellar avg mol wt 6150 |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze desiccated (hygroscopic) |
| 聚集数 | 10 |
| 颜色 | white |
| CMC | 6 - 10 mM 6 mM (20-25℃) |
| 转变温度 | cloud point >100 ℃ |
| 溶解性 | water: 1.0 M |
| 异质活性 | DNase, RNase, and Protease, none detected |
| 运输 | ambient |
| 储存温度 | 15-25℃ |
| SMILES string | C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C |
| InChI | 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1 |
| InChI key | UMCMPZBLKLEWAF-RFCNGIAKSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
