VCaP Cell Line human

半岛bd体育手机客户端 说明

细胞系来源

Human Prostate Cancer Metastasis

细胞系描述

This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitivein vitroandin vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19

培养基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm²using 0.25% trypsin; 5% CO₂; 37℃. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human prostate (metastasis)
生长模式	Adherent
核型	Refer to reference (Korenchuk et al., 2001)
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Androgen receptor
technique(s)	cell culture | mammalian: suitable
相关疾病	metastasis
运输	dry ice
储存温度	?196℃