半岛bd体育手机客户端 说明
细胞系来源
Hamster Chinese lung
细胞系描述
Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).
应用
Mutagenesis and mammalian cell genetic studies
培养基
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm2; 5% CO2; 37℃ using 0.25% trypsin/EDTA.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | hamster lung |
| 生长模式 | Adherent |
| 核型 | 2n = 22 |
| 形态学 | Fibroblast |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
