TM3

半岛bd体育手机客户端 说明

细胞系来源

Mouse BALB/c testis, Leydig cell

细胞系描述

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

应用

Hormone studies

培养基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37?C.

其他说明

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半岛bd体育手机客户端 性质

生物来源	mouse testis
生长模式	Adherent
核型	Not specified
形态学	Not specified
半岛bd体育手机客户端	Prostaglandin F2a
受体	Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃