RT112/84

半岛bd体育手机客户端 说明

细胞系来源

Human bladder carcinoma epithelial

细胞系描述

A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.

应用

RT112/84 cancer cell line has been used to study the cytotoxic effect of lipoic acid and calcium hydroxycitrate concentrations on RT112/84 cells.
Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 11,13
D5S818: 10,13
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 14,17

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

半岛bd体育手机客户端 性质

生物来源	human bladder
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃