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半岛bd体育手机客户端 说明

细胞系来源

American Opossum, kidney

细胞系描述

Derived from proximal tubule of a kidney taken from an adult female opossum. Cells retain proximal tubule function. Possesses the following receptors: α 2 - adrenergic, insulin, serotonin, parathyroid hormone and atrial natriuretic factor.

应用

Kidney function studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	opossum kidney
生长模式	Adherent
核型	Not specified
形态学	Not specified
半岛bd体育手机客户端	Not specified
受体	Insulin, serotonin, alpha 2-adrenergic parathy. Hormone
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃