半岛bd体育手机客户端 说明
细胞系来源
Rat kidney fibroblast
细胞系描述
Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.
应用
Contact inhibition and transformation studies
培养基
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. The cells should be maintained sub-confluent, otherwise they will transform.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | rat kidney |
| 生长模式 | Adherent |
| 核型 | Not specified |
| 形态学 | Fibroblast |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
