NIH-3T3 4-2

半岛bd体育手机客户端 说明

一般描述

Product has lost contact inhibition

细胞系来源

Mouse fibroblast

细胞系描述

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

应用

Virus studies: Moloney MuLV, retroviruses

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	mouse skin
生长模式	Adherent
核型	Not specified
形态学	Fibroblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃