NB4 1A3

半岛bd体育手机客户端 说明

细胞系来源

Mouse C-1300 Neuroblastoma

细胞系描述

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternatein vivoand in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

应用

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

培养基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	mouse nerve
生长模式	Adherent
核型	Unstable
形态学	Neuroblast
半岛bd体育手机客户端	Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃