半岛bd体育手机客户端 说明
细胞系来源
Mouse Sp2/0 x Mouse BALB/c spleen
细胞系描述
The antibody recognises human p62c-myc gene product and is specific for C-terminal peptide G. The antibody failed to immunoprecipitate p62 c-myc, but recognised the imunoblotted protein. Compared to other hybridomas specific to this antigen (Mycl-8F9, Mycl-3C7, CT9-B7, CT14-B2) Mycl-9E10 is the most sensitive probe for immunoblots. Conformation of antigen is critical for antibody recognition. No reported cross-reaction, with chicken p110gag-myc protein or mouse c-myc protein. This cell line is available as a growing culture only to customers in Europe.
免疫原
A synthetic peptide, corresponding to residues 408-432(C-terminus of Peptide G) of human p62c-myc sequence, conjugated to KLH
培养基
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. Cells are slow to grow. This cell line is available as a growing culture only to customers in Europe.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
半岛bd体育手机客户端 性质
生物来源 | Mouse x Mouse Hybridoma |
ImmunoDon | BALB/c mouse spleen |
骨髓瘤 | Sp2/0 |
反应性 | Anti-c-Myc gene product, human |
technique(s) | cell culture | mammalian: suitable |
同位素/亚型 | IgG1, kappa |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich