半岛bd体育手机客户端 说明
细胞系来源
Mink lung
细胞系描述
Derived from trypsinised lungs of several foetuses of Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.
应用
Virus focus forming assays: MSV, feline sarcoma virus
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
半岛bd体育手机客户端 性质
生物来源 | mink lung |
生长模式 | Adherent |
核型 | 2n = 30; diploid and pseudodiploid |
形态学 | Epithelial |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich