MDCK

半岛bd体育手机客户端 说明

细胞系来源

Canine Cocker Spaniel kidney

细胞系描述

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

应用

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

基本信息

eCl@ss

32190102

半岛bd体育手机客户端 性质

生物来源	dog kidney
生长模式	Adherent
核型	2n = 78
形态学	Epithelial-like
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃