IEC 6

半岛bd体育手机客户端 说明

细胞系来源

Rat small intestine epithelial

细胞系描述

Normal rat small intestine epithelial cells which synthesise fibronectin and collagen. Growth is inhibited by cortisol. Cells possess cell surface antigens specific for intestinal epithelial cellsin vivo. Capable of at least 10 population doublings.

应用

Small bowel biology

培养基

DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	rat intestine
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃