CTLL-2

半岛bd体育手机客户端 说明

细胞系来源

Mouse C57b1/6 T cell

细胞系描述

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

应用

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.

培养基

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9 x 10⁵cells/ml; 5% CO₂; 37℃. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵cells/ml.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	mouse blood
生长模式	Suspension
核型	Not specified
形态学	Lymphoblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃