CAD cell line from mouse

半岛bd体育手机客户端 说明

细胞系来源

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

细胞系描述

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

应用

Study of neuronal differentiation

培养基

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴cells/cm²Cells should be dislodged by gentle pipetting; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	mouse nerve
描述	Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
生长模式	Adherent
核型	Not specified
形态学	Neuronal
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃