半岛bd体育手机客户端 说明
细胞系来源
Mouse pre-B lymphocyte
细胞系描述
Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.
培养基
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37℃.
其他说明
Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
半岛bd体育手机客户端 性质
| 生物来源 | mouse blood |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Lymphoblastoid |
| 半岛bd体育手机客户端 | Cytoplasmic Immunoglobulin |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
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