半岛bd体育手机客户端 说明
细胞系来源
Rabbit Cornea
细胞系描述
Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
应用
Virus studies: Rubella virus
培养基
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2using 0.25% trypsin 0.02% EDTA; 5% CO2, 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | rabbit eye |
生长模式 | Adherent |
核型 | 2n = 44 |
形态学 | Epithelial |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich