半岛bd体育手机客户端 说明
细胞系来源
Mouse C3H/An connective tissue
细胞系描述
From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.
应用
Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | mouse connective tissue |
| 生长模式 | Adherent |
| 核型 | 2n = 40 |
| 形态学 | Fibroblast |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
