半岛bd体育手机客户端 说明
细胞系来源
Mouse C3H/An liver
细胞系描述
Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
应用
Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)
培养基
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37℃. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
半岛bd体育手机客户端 性质
| 生物来源 | mouse liver |
| 生长模式 | Adherent |
| 核型 | Not specified |
| 形态学 | Epithelial |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
Sigma-Aldrich
m.cnreagent.com
