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MH-S

货号
CB_95090612
规格纯度
95090612
价格
4633 *本价格含增值税费
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半岛bd体育手机客户端 名称:
MH-S
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

细胞系来源

Mouse alveolar macrophage, SV40 transformed

细胞系描述

The MH-S cell line was obtained from a 7 week-old male BALB/c J mouse by bronchoalveolar lavage followed by SV-40 transformation of an adherent cell enriched population of alveolar macrophages. Many properties of alveolar macrophages are expressed with the cells being phagocytic, esterase positive and peroxidase negative. The intracellular T-antigen and cell surface Ia and Mac-1 antigens are expressed as well as Fc receptors. IL-1 is constitutively expressed and can be increased by stimulation with lipopolysaccharides (LPS). A cellulose dependant suppression of the in vitro plaque-forming cell (PFC) i-espouse by MH-S cells has also been reported.

应用

Phagocytosis studies, plaque assays, IL-1 assays

培养基

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源 mouse lung (alveoli)
生长模式 Adherent
核型 Not specified
形态学 Macrophage
半岛bd体育手机客户端 Not specified
受体 Not specified
technique(s) cell culture | mammalian: suitable
运输 dry ice
储存温度 ?196℃

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