半岛bd体育手机客户端 说明
细胞系来源
Mouse alveolar macrophage, SV40 transformed
细胞系描述
The MH-S cell line was obtained from a 7 week-old male BALB/c J mouse by bronchoalveolar lavage followed by SV-40 transformation of an adherent cell enriched population of alveolar macrophages. Many properties of alveolar macrophages are expressed with the cells being phagocytic, esterase positive and peroxidase negative. The intracellular T-antigen and cell surface Ia and Mac-1 antigens are expressed as well as Fc receptors. IL-1 is constitutively expressed and can be increased by stimulation with lipopolysaccharides (LPS). A cellulose dependant suppression of the in vitro plaque-forming cell (PFC) i-espouse by MH-S cells has also been reported.
应用
Phagocytosis studies, plaque assays, IL-1 assays
培养基
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | mouse lung (alveoli) |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Macrophage |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich