半岛bd体育手机客户端 说明
细胞系来源
Mouse kidney cortical collecting duct, SV40 transformed
细胞系描述
Derived from the cortical collecting duct (CCD) of a mouse, transgenic for the early region of SV40 (Tg(SV40E)Bri/7). They express many characteristics of the CCD like epithelial morphology and CCD-specific antigens. M-1 cells grown on permeable supports exhibited a high transepithelial resistance concomitant with the development of a lumen-negative transepithelial potential difference. The M-1 cell line and sub-clones exhibit principal cell (PC) functions and ?-intercalated cell (?-ICC) functions, both typical for the heterogeneous epithelium found in the renal collecting duct. M-1 cells and sub-clones show a heterogeneous expression of PC and ?-ICC antigens with 5-10% exhibiting a dual PC/?-ICC phenotype.
应用
Kidney cell studies, differentiation studies.
培养基
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μm Dexamethasone (DXMT) + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. Prolonged incubation with trypsin may be necessary for subculture.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
半岛bd体育手机客户端 性质
生物来源 | mouse kidney |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Epithelial-like |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich