L6.G8.C5

半岛bd体育手机客户端 说明

细胞系来源

Rat skeletal muscle myoblast

细胞系描述

A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.

其他说明

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半岛bd体育手机客户端 性质

生物来源	rat muscle
生长模式	Adherent
核型	Not specified
形态学	Fibroblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃