HTC

半岛bd体育手机客户端 说明

细胞系来源

Rat hepatoma

细胞系描述

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

应用

Tumourigenicity and enzymatic studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	rat liver
生长模式	Adherent
核型	2n = 66, hypertriploid
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Dexamethasone phosphate; hydrocortisone
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃