H-4-II-E

半岛bd体育手机客户端 说明

细胞系来源

Rat hepatoma Reuber H35

细胞系描述

Exhibits high arylhydrocarbon hydroxylase inducibility and is used to detect picogram levels of planar polychlorinated organic compounds in food extracts and other environmental samples.

应用

Hormone induced enzymatic studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	rat liver
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃