GH3

半岛bd体育手机客户端 说明

细胞系来源

Rat Wistar-Furth pituitary tumour

细胞系描述

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

应用

Hormone studies

培养基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

其他说明

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半岛bd体育手机客户端 性质

生物来源	rat brain
生长模式	Adherent
核型	Hyperdiploid, modal no. 60
形态学	Epithelial
半岛bd体育手机客户端	Growth hormone, prolactin
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃