半岛bd体育手机客户端 说明
细胞系来源
Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147
细胞系描述
Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.
培养基
DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 ℃. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
半岛bd体育手机客户端 性质
| 生物来源 | mouse unknown/unspecified |
| 生长模式 | Suspension |
| 核型 | Not specified |
| 形态学 | Not specified |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
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