Clone M-3

半岛bd体育手机客户端 说明

细胞系来源

Mouse (CxDBA)F1 melanoma

细胞系描述

Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.

应用

Cloning studies

培养基

Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	mouse skin
生长模式	Adherent
核型	Hypertetraploid
形态学	Epithelial
半岛bd体育手机客户端	Melanin
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃