半岛bd体育手机客户端 说明
细胞系来源
Hamster Syrian kidney
细胞系描述
Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.
应用
Virus replication studies
培养基
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37℃. Cells need to be grown in a techni-stirrer or they may start to re-attach.
其他说明
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半岛bd体育手机客户端 性质
| 生物来源 | hamster kidney |
| 生长模式 | Suspension |
| 核型 | 2n = 44, pseudodiploid |
| 形态学 | Not specified |
| 半岛bd体育手机客户端 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | ?196℃ |
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