半岛bd体育手机客户端 说明
细胞系来源
Hamster Syrian kidney
细胞系描述
BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
应用
Transformation and virus studies
培养基
DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | hamster kidney |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Fibroblast |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | ?196℃ |