BAE-1

半岛bd体育手机客户端 说明

细胞系来源

Bovine aortic endothelium

细胞系描述

Derived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin; 5% CO₂; 37℃. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevail

其他说明

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半岛bd体育手机客户端 性质

生物来源	bovine cardiovascular
生长模式	Adherent
核型	59 or 60
形态学	Endothelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃