B50

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细胞系来源

Rat nervous tissue neuronal

细胞系描述

Ethyl nitrosourea induced tumour cell line. Neuronal cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.

应用

Neurobiological studies

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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