AtT20

半岛bd体育手机客户端 说明

细胞系来源

Mouse LAF1 pituitary gland tumour

细胞系描述

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

应用

Hormone studies

培养基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

传代培养常规

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	mouse brain
生长模式	Aggregates in suspension
核型	Diploid
形态学	Small round cells growing as large aggregates
半岛bd体育手机客户端	Adrenocorticotropic hormone (ACTH)
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃