半岛bd体育手机客户端 说明

应用

RPMI-1640培养基被用于以下应用：

• 用于细胞培养基：在补充有其他组分的RPMI 1640培养基中培养小鼠胸腺瘤EL-4细胞
• 用于脾细胞的分枝杆菌感染(在无抗生素的RPMI 1640培养基中)
• 用于细胞分离和培养
• 用于淋巴细胞分离和培养、ELISPOT测定、间接体内增殖测定所用的培养基中(作为组分之一)
• 用于gp39基因表达和CD40-免疫球蛋白结合试验所用的培养基中
• 用于制备抗真菌剂

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

数量

按照每升培养基10.4克粉末配制。

重悬

补充2.0 g/L碳酸氢钠。

其他说明

酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。

基本信息

MDL编号	MFCD00217820
NACRES	NA.75

半岛bd体育手机客户端 性质

无菌性	sterile
形式	powder
technique(s)	cell culture | mammalian: suitable
web metadata keyword.default	classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells
组分	phenol red: no NaHCO3: no L-glutamine: yes sodium pyruvate: no HEPES: no
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable