Anti-CD3 Mouse mAb (UCHT1)

半岛bd体育手机客户端 说明

一般描述

Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.
Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

免疫原

human thymocytes/Sezary T cells

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (5 ?g/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

警告

Toxicity: Standard Handling (A)

其他说明

Thibault, G. and Bardos, P. 1995.J. Immunol.154,3814.
Beverley, P.C., and Callard, R.E. 1981.Eur. J. Immunol.11,329.
This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 ?g/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	UCHT1, monoclonal
测定	>95% (immunoglobulin, SDS-PAGE)
形式	liquid
包含	0.5 mg/mL gentamycin sulfate as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable