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一般描述

Tris hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

应用

所有缓冲液的pH值都与温度和浓度有关。对于Tris缓冲液，降温每℃其pH值约增加0.03单位，每10倍稀释其pH降低0.03-0.05单位。
对于精确的应用，请使用带有玻璃/甘汞组合电极并仔细校准过的pH计。
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per ℃ decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Tris hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.

法律信息

Trizma is a registered trademark of sigma-aldrich Co. LLC

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