Protein A soluble fromStaphylococcus aureus(Cowan strain)

半岛bd体育手机客户端 说明

一般描述

Protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. This protein is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel alpha-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also associates with IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat.
Protein A is involved in regulating anti-tumor, toxic, and carcinogenic functions. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.

应用

Protein A may be conjugated with various reporter molecules such as fluorescent dyes (FITC), enzyme markers (peroxidase, beta-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates may be used to detect immunoglobulins using immunochemical assays such as western blotting, immunohistochemistry, and ELISA. In addition, protein A may also be immobilized on a solid supports like agarose or acrylic beads for the purification of polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays. Furthermore, protein A has been used for affinity chromatography and SDS-PAGE assays.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00132106
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	100
生物来源	Staphylococcus aureus
偶联物	unconjugated
形式	powder
species reactivity	human, mouse, monkey, rabbit, guinea pig, cat, dog, pig
technique(s)	ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
颜色	white
容量	~10 mg/mg binding capacity (human IgG)
溶解性	H2O: 1 mg/mL, clear, colorless
UniProt登记号	P02976
储存温度	2-8℃
Gene Information	Staphylococcus aureussubsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)