Caspase-9, Human, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant, human caspase-9 fused at the N- and C- termini to a His?Tag sequence and expressed inE. colias a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.

包装

100 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.

外形

Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH?)?SO?

重悬

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.

其他说明

Qin, H., et al. 1999.Nature399,549.
Li, P., et al. 1997.Cell91,479.
Salvesen, G.S., et al. 1997.Cell91,443.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
测定	≥70% (SDS-PAGE)
形式	lyophilized
specific activity	≥400 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
溶解性	PBS with 15% glycerol: 1 units/μL
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1