半岛bd体育手机客户端 说明
一般描述
Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1Vibrio Harveyigenomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
SinceV. Harveyiis not a typical resident of the human microbiota it can serve as a spike-in standard in human microbiota genomics workflow analysis.
V. harveyiis a gram negative, rod-shaped, motile, heterotrophic marine luminous pathogen bacterium that widely inhabits natural aquatic environments. It belongs to the family Vibrionaceae of class Gammaproteobacteria 2, known as a serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp in aquaculture 2,3.
V. harveyihas the ability to regulate gene expression by quorum sensing. Current research 4,5,6 demonstrates 3 regulatory molecules that mediate quorum sensing in inV. harveyi; homoserine lactone (HAI-1) , a furanosyl borate diester (AI-2) and cholerae autoinducer 1 (CAI-1). The quorum sensing pathway that regulates bioluminescence, biofilm formation and other virulence factors inV. harveyiis very similar to the pathway that regulates virulence factor expression inVibrio cholerae, 7, 8 makingV. harveyi an important model system for human health research.
应用
Suitable as a standard for PCR, Sanger and next generation sequencing (NGS).
特点和优势
外形
The genomic DNA is provided in at ≥10 ng/μl concentration in TE buffer pH 8.0.
其他说明
It is recommended to avoid freeze thaw cycles of this product
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 形式 | liquid |
| 组成 | , 10 ng/μL |
| 运输 | ambient |
| 储存温度 | ?20℃ |
Sigma-Aldrich
m.cnreagent.com
