半岛bd体育手机客户端 说明

一般描述

Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH₂terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys₁₃₁- Ser₁₃₂resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His₄₆and Ser₁₈₃at the active site. The pH optimum of trypsin is 7 - 9.

应用

Trypsinogen from bovine pancreas is suitable for use in:

• the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy
• tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometer
• the secondary structure analysis of proteins by infrared (IR) spectroscopy
• SDS-PAGE as a molecular weight standard (24kDa)

包装

1 g in glass bottle
250 mg in glass bottle

生化/生理作用

Phytic acid complexed with calcium has been shown to increase the secretion of trypsinogen unable to be cleaved for activation. It also reduced the stabilization effect of calcium on activated trypsin. The active form of trypsinogen, referred to as trypsin, cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. It also hydrolyzes ester and amide linkages of synthetic derivatives of amino acids such as, benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl-L-arginine methyl ester (TAME), etc.

单位定义

One BAEE unit is equal to a ΔA₂₅₃of 0.001 per min with BAEE as substrate at pH 7.6 at 25 ℃ and a reaction volume of 3.2 mL (1 cm light path).

基本信息

EC 号	232-651-3
NACRES	NA.56

半岛bd体育手机客户端 性质

质量水平	200
生物来源	bovine pancreas
测定	85-100% (UV)
形式	essentially salt-free, lyophilized powder
specific activity	≥10,000 BAEE units/mg protein (E 1%/280 , after activation to trypsin)
分子量	23,981 Da by calculation
technique(s)	mass spectrometry (MS): suitable
溶解性	H2O: soluble 10 mg/mL
UniProt登记号	Q29463
储存温度	?20℃
Gene Information	bovine ... TRYP8(282603)

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H334
预防措施声明	P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Faceshields, Gloves, type N95 (US)