半岛bd体育手机客户端 说明

一般描述

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.
This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_ex= 490/λ_em= 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

特点和优势

Compatible with high-throughput handling systems.

适用性

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

原理

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λ_Ex= 490/λ_Em= 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

基本信息

NACRES

NA.84

半岛bd体育手机客户端 性质

用途	(Fluorometric MDR Assay)
detection method	fluorometric
储存温度	?20℃
Gene Information	human ... ABCB1(5243), ABCC2(1244) mouse ... ABCC2(12780) rat ... abcc2(25303)

安全信息

储存分类代码

10 - Combustible liquids