半岛bd体育手机客户端 说明

一般描述

Affinity isolated antigen specific antibody is obtained from goat anti-mouse IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, that do not specifically bind to mouse IgG. Goat anti-Mouse IgG is conjugated to Sigma Alkaline Phosphatase, Type VII-S (Product No. P 5521) by protein crosslinking with 0.2% glutaraldehyde. Anti-Mouse IgG (Whole Molecule) is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP), versus mouse IgG and normal mouse serum, prior to conjugation. Prior to conjugation, the antibody is found to be reactive with mouse IgG1, IgG2a, IgG2b, and IgG by Ouchterlony Double Diffusion (ODD). Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

特异性

mouse IgG1, IgG2a, IgG2b, and IgG
Binds all mouse Igs.

免疫原

Purified mouse IgG

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blot analysis of nuclear or mitochondrial protein extracts were performed using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at a 1:5000 dilution in 5%BSA/TBS for 1 hour at room temperature. 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (Sigma) was used for the substrate.
在含有终浓度为0.5M NaCl的TBS/Tween中，使用碱性磷酸酶缀合的山羊抗小鼠IgG(μg/ ml)作为二抗，对支气管肺泡液中的表面活性蛋白A进行检测。

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00162640
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	goat
偶联物	alkaline phosphatase conjugate
抗体形式	affinity isolated antibody
antibody product type	secondary antibodies
克隆	polyclonal
形式	buffered aqueous solution
technique(s)	direct ELISA: 1:7,000-1:21,000
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2