半岛bd体育手机客户端 说明
一般描述
Protein kinase regulated by RNA (PKR) is an interferon-inducible ser/thr protein kinase, activated by stress signals and double-stranded RNA (dsRNA). In response to viral infection, PKR is activated by dsRNA-mediated dimerization and autophosphorylation. α-subunit of protein synthesis initiation factor 2 (eIF-2α) is well-studied substrate of PKR. Activation by PKR leads to phosphorylation on Ser51 that result in inhibition of translation. Human PKR contains at least 15 autophosphorylation sites. But phosphorylation on threonine 446 and 451 in the PKR activation loop is requiredin vivoandin vitrofor high-level kinase activity. PKR is implicated in signalling pathways that involve NF-κB, p38, and IRF-3 and is the key effector in IFN-induced anti-viral responses, apoptosis and protein synthesis.
Anti-Phospho-PKR [pThr451] specifically recognizes PKR phosphorylated on threonine 451 (65-68 kDa). The antibody detects human and mouse PKR [pThr451].
免疫原
synthetic phosphopeptide derived from the region of PKR that is phosphorylated on threonine 451.
应用
Anti-phospho-PKR (pThr451) antibody may be used for detection by immunoblotting at a working dilution of 1:1,000 using human HeLa cells stimulated with IFN·γ and calyculin. For detection by immunoblotting in human HeLa cells, a working concentration of 0.1-1.0 μg/mL is recommended. Detection of phospho-PKR (pThr451) was done in peritoneal macrophages obtained from thioglycolate-treated mice and human peripheral blood mononuclear cells at a working dilution of 1:500
外形
Supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide. The amount of the reagent is sufficient for 10 blots.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD03455798 |
NACRES | NA.44 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous glycerol solution |
分子量 | antigen 65-68 kDa |
species reactivity | human, mouse |
technique(s) | immunoblotting: suitable using human HeLa cells stimulated with IFNγ and calyculin |
UniProt登记号 | P19525 |
运输 | wet ice |
储存温度 | ?20℃ |
Gene Information | human ... EIF2AK2(5610) mouse ... Eif2ak2(19106) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich