RK-13 Cell Line from rabbit

半岛bd体育手机客户端 说明

细胞系来源

Rabbit kidney

细胞系描述

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

应用

Virus studies

培养基

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37?C; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

其他说明

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基本信息

eCl@ss

32190102

半岛bd体育手机客户端 性质

生物来源	rabbit kidney
生长模式	Adherent
核型	2n=44, stem-line = 56 (=22%)
形态学	Epithelial-like
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃