L929/A

半岛bd体育手机客户端 说明

细胞系描述

This Adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue number 85011425) to increasing concentrations of doxorubicin in-vitro. L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

培养基

RPMI 1640 + 10% Fetal Bovine Serum (FBS) + 2mM L-Glutamine + 0.086 uM Doxorubicin hydrochloride.

传代培养常规

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures vessels at 1-2 x 10⁴cells/cm². Cultures incubated in a humidified 5% CO₂/95% air incubator at 37^oC.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

半岛bd体育手机客户端 性质

生物来源	mouse adipose tissue
描述	Mouse C3H/An, connective tissue, Adriamycin,Doxorubicin, drug resistant
生长模式	Adherent
形态学	Fibroblast
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃