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位置: 首页> 品牌> Sigma-Aldrich> Transcreener?EPIGEN Methyltransferase Assay

Transcreener?EPIGEN Methyltransferase Assay

品牌
Sigma-Aldrich
货号
3017
包装型号
规格纯度
价格
18172.7 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Transcreener ?EPIGEN Methyltransferase Assay
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The Transcreener?EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener?AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener?AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener?AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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数量

3017-1K = 1,000 assay, 384-well
3017-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

基本信息

NACRES NA.84

半岛bd体育手机客户端 性质

运输 dry ice
储存温度 ?20℃

Sigma-Aldrich

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