LB Broth with agar (Luria low salt)

半岛bd体育手机客户端 说明

一般描述

Luria′s low salt LB is a microbial growth medium used for the cultivation ofE. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

应用

Suitable for non-selective cultivation ofE. colistrains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^?.
LB Broth with agar (Luria low salt) has been used for culturingE. coliDH10B cells and in the isolation of diazotrophic bacteria.

特点和优势

Luria′s low salt LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

制备说明

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121℃.
4. Cool to 50℃ prior to dispensing into petri dishes.

法律信息

Zeocin is a registered trademark of Cayla Sarl

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
无菌性	non-sterile
形式	powder
组成	Agar, 15 g/L NaCl, 0.5 g/L Tryptone, 10 g/L Yeast Extract, 5 g/L
pH范围	6.8 - 7.2 (3.05% solution)
application(s)	food and beverages
储存温度	room temp
适用性	nonselective forEscherichia coli nonselective for coliforms

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable