Chondroitinase ABC from Proteus vulgaris

半岛bd体育手机客户端 说明

一般描述

Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.

应用

• Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
• Neural regeneration research
• Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
• Quantitation of glucosaminoglycans (mucopolysaccharides)

特点和优势

• Low Endotoxin
• BSA Free
• Recombinant
• Endolytic component only, does not contain exolytic enzyme.
• Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate

组分

Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water

相容性

pH optimum:

• pH 8.0 (chondroitin sulfate)
• pH 6.8 (hyaluronic acid)

Temperature optimum: 37 ℃
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn ²⁺Ni ²⁺, Fe ²⁺and Cu ²⁺

单位定义

1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 ℃, using chondroitin sulfate from shark cartilage (C4384) as substrate.

储存及稳定性

Store the product at -70 ℃. When stored unopened at -70 ℃, the buffer is stable for at least two years.

其他说明

This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.

半岛bd体育手机客户端 性质

质量水平	200
specific activity	50-250 units/mg protein
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 3