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位置: 首页> 品牌> Sigma-Aldrich> Cas9 Hygromycin Lenti Plasmid

Cas9 Hygromycin Lenti Plasmid

品牌
Sigma-Aldrich
货号
CAS9HYGROVP
包装型号
规格纯度
价格
2486 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Cas9 Hygromycin Lenti Plasmid
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a hygromycin resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Hygro) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-Hygro lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Manufacture of Cas9 expressing Lentiviral Particles

特点和优势

  • Highly specific and highly active
  • Sequence verified
  • Ready to use purified plasmid DNA

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacteriumStreptococcus pyogeneshas been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

法律信息

CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses

半岛bd体育手机客户端 性质

重组 expressed inE. coli
包装 vial of 50 μL
浓度 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s) CRISPR
selection blasticidin
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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