半岛bd体育手机客户端 说明
一般描述
This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a hygromycin resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Hygro) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-Hygro lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.
To order gRNA in any format click here
应用
Functional Genomics/Target Validation
特点和优势
原理
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacteriumStreptococcus pyogeneshas been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
法律信息
CRISPR Label License
Lentiviral, WPRE, and Evrogen Licenses
半岛bd体育手机客户端 性质
重组 | expressed inE. coli |
包装 | vial of 50 μL |
浓度 | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
application(s) | CRISPR |
selection | blasticidin |
运输 | dry ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich