Lenti CRISPR Universal Non-Target Control#2 Transduction Particles (LV04 vector)

半岛bd体育手机客户端 说明

一般描述

The Lenti CRISPR Non-Target Control#2 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#2 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#2 uses a dual-component system consisting of U6-driven guide RNA (non-target#2) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

应用

功能性基因组学研究/筛选/靶标验证

其他说明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

法律信息

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

基本信息

NACRES

NA.51

半岛bd体育手机客户端 性质

质量水平	200
包装	vial of 200 μL
浓度	(1x106TU/mL (via p24 titering assay))
application(s)	CRISPR
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable